Microscope Parts And Functions
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Here are the parts of a microscope:
- Eyepiece: The eyepiece (sometimes called the 'ocular') is thelens of the microscope closest to the eye that you look through. Itis half of the magnification equation (eyepiece power multiplied byobjective power equals magnification), and magnifies the image madeby the objective lens... sometimes called the virtual image.Eyepieces come in many different powers. One can identify whichpower any given eyepiece is by the inscription on the eyecup of thelens, such as '5x,' '10x,' or '15X.' Oculars are also designed withdifferent angles of view; the most common is the wide field(W.F.).
- Eyepiece Holder: This simply connects the eyepiece to themicroscope body, usually with a set-screw to allow the user toeasily change the eyepiece to vary magnifying power.
- Body: The main structural support of the microscope whichconnects the lens apparatus to the base.
- Nose Piece: This connects the objective lens to the microscopebody. With a turret, or rotating nose piece as many as fiveobjectives can be attached to create different powers ofmagnification when rotated into position and used with the existingeyepiece.
- Objective: The lens closest to the object being viewed whichcreates a magnified image in an area called the 'primary imageplane.' This is the other half of the microscope magnificationequation (eyepiece power times objective power equalsmagnification). Objective lenses have many designs and qualitieswhich differ with each manufacturer. Usually inscribed on thebarrel of the objective lens is the magnification power and thenumerical aperture (a measure of the limit of resolution of thelens).
- Focusing Mechanism: Adjustment knobs to allow coarse or fine(hundredths of a millimeter) variations in the focusing of thestage or objective lens of the microscope.
- Stage: The platform on which the prepared slide or object to beviewed is placed. A slide is usually held in place by spring-loadedmetal stage clips. More sophisticated high-powered microscopes havemechanical stages which allow the viewer to smoothly move the stagealong the X (horizontal path) and Y (vertical path) axis. Amechanical stage is a must for high-power observing.
- Illumination Source: The means employed to light the object tobe viewed. The simplest is the illuminating mirror which reflectsan ambient light source to light the object. Many microscopes havean electrical light source for easier and more consistent lighting.Generally electrical light sources are either tungsten orfluorescent, the fluorescent being preferred because it operates ata cooler temperature. Most microscopes illuminate from underneath,through the object, to the objective lens. On the other hand,stereo microscopes use both top and bottom illumination.
- Base: The bottom or stand upon which the entire microscoperests or is connected.
Microscope Parts & Functions. A lens mounted in or below the stage whose purpose is to focus or condense the light onto the specimen.
Compound light microscope:
- Eyepiece (ocular lens): The part you look through. It has alens that magnifies the object, usually by ten times (10x). Themagnifying power is engraved on the side of the eyepiece.
- Tube: Holds the eyepiece and the objective lenses at the properworking distance from each other.
- Revolving nosepiece: Rotating disk holds two or more objectivelenses. Turn it to change lenses. Each lens clicks into place.
- Objective lenses: Magnify the object. Each lens has a differentpower of magnification, such as 10x, 40x, and 100x.The magnifyingpower is engraved on the side of each objective lens. Be sure youcan identify each lens. For example, the low-power objective lensis usually 10x.
- Fine-adjustment knob: Use with medium- and high-powermagnification to bring the object into sharper focus.
- Coarse-adjustment knob: Moves the tube or stage up or down andbrings the object into focus. Use it only with the low-powerobjective lens.
- Stage Supports: the microscope slide. Clips hold the slidesinto position. A hole in the center of the stage allows the lightfrom the light source to pass through the slide.
- Condenser lens: Directs light to the object being viewed.
- Diaphragm: Use this to control the amount of light reaching theobject being viewed.
- Light source: Shining a light through the object being viewedmakes it easier to see the details. (Your microscope might have amirror instead of a light. If it does, you will adjust it to directlight through the lens.)
Overall, the function of a microscope is to view specimens toosmall to be viewed by the human eye.
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